Biological and chemical interest in selenium: a brief historical account

This review presents a brief account of the discovery, importance, and use of selenium. Based in the importance of selenoproteins, their mechanism of reaction with the participation of selenium, as a selenol (-SeH) group, are indicated. Since the selenol group is the softest nucleophile center found in life, a brief discussion about the synthesis and possible antioxidant and selenoprotein mimetic effects of the organoselenium compounds that can generate the selenol group is presented


1.Introduction 1.1 The selenol group: the softest of the nucleophiles in cell biology
2][3] Nowadays, we have a good understanding about its chemistry but only a partial knowledge about its role in the living cell -physiology or toxicology.][6][7][8][9][10][11][12][13][14] The selenol (or selenohydryl) is an analog of the thiol (or sulfhydryl) group of cysteine and the or hydroxyl group found in serine (Figure 1).These functional groups have different roles in cell physiology and here we will focus on the properties of the selenol group.The general chemical structure of these amino acids is depicted in Figure 1.Serine and cysteine can be found in body fluid as free amino acids in the low millimolar range (e.g., 0.05-0.2mmoL/L). 12In contrast, selenocysteine is stable only inside the structure of selenoproteins and it cannot be detected in biological fluids as a free amino acid.The selenol group has lower redox potential than the analogous thiol group. 13,14Serine has 6 codons, cysteine has 2 codons and selenocysteine has only one codon (UGA, which normally is the termination codon in mRNA molecules coding a protein).The incorporation of selenocysteine in selenoproteins is co-translational and involves the participation of structural factors both from the mRNA structure and from specific proteins that interact in the ribosomes with the UGA codon 5 (Table 3).][10][11]14,[25][26][27][28][29][30] A schematic representation of the chemical reactions catalyzed by well-studied selenoenzymes is depicted in Table 4. Table 4. Diagrammatic representation of the reactions of the seleno groups in mammalian cells 27,28 .
For commentary and explanation see the paragraph that follows.
No. The reaction of peroxides with the selenol group can form either H2O or alcohols.In the living cell, reaction 1 indicated for R 1 -SeH is catalyzed by isoforms of glutathione peroxidase (GPx).The GPx isoforms reduce peroxides, using reduced glutathione (GSH) as the source of reducing equivalents.Since H2O2 and organic peroxides (ROOH) can react with Fe 2+ , forming extremely reactive intermediates (for instance, • OH and ROO • ), the enzyme protects cells from the oxidative stress induced by peroxide metabolites.In reaction 2, the Se atom of the selenenic acid is attacked by an S from the thiol group of a reduced GSH forming the -S-Sebond.This type of bond is central in the biological chemistry of selenium and illustrates the importance of sulfur-selenium interaction in cell biology.In reaction 3, a second molecule of GSH attacks the sulfur in the intermediate -S-Se-, regenerating the R 1 -SeH.The reactions catalyzed by the thioredoxin reductase (TrxR) isoforms are represented in reactions 4 and 5: first the -S-Se-bond is reduced by two vicinal thiol groups, then the selenol and thiol group of TrxR reacts with the disulfide bond (-S-S-) found in the oxidized Trx, reducing it to the reduced Trx.In the methionine sulfoxide reductase (MsrB) (reactions 6 to 8), the selenol group of the enzyme is supposed to interact with the sulfur atom of the sulfoxide, the selenol group is oxidized to a selenenic acid and the methionine is regenerated (reaction 6).The thiol group of MsrB, which is in close proximity to a selenenic group in the active center of the enzyme, attacks this oxidized group, releasing H2O and forming a -S-Se-inside the active center of MsrB (reaction 7).The oxidized enzyme (-S-Se-) can be regenerated by reduced Trx (reaction 8).The sequence of reactions 4-9 demonstrate that the substrate of TrxR (i.e., the Trx) is important to maintaining the MsrB in its active form and indicate an intricate relationship among the selenoenzymes.The reactions 1-8 indicate that sulfur-selenium interactions are fundamental for selenoprotein physiological roles.Reactions 9 and 10 represent the steps of the reaction catalyzed by iodothyronine deiodinase (Dio) isoforms.In the first step, the selenol group of Dio attacks the C-I bond, forming the intermediate -Se-I.In the second step, an endogenous thiol (still not identified) regenerates the active Dio.

Reagents
In this mini review, we will discuss basic aspects of the role of selenoproteins in cell physiology but will not give details about the discoveries that led to the establishment of selenocysteine as the twenty first amino acid.For a comprehensive view of this exciting field of selenium biology, we refer the reader to several important reviews and references (for instance, refs 31-33).

The essentiality of selenium to life
From the chemical point of view, life is based on carbon and on other relatively light elements bound covalently to carbon or existing as free ions in the aqueous environment of living cells (Figure 2).The exceptions are lithium, fluorine and beryllium that probably because of their size and reactivity could not be selected to exist in the living cell as ions.Iodine is the heaviest element found in organic molecules in mammals, but it has a limited role as a component of the thyroid hormones 3,3',5,5'-tetraiodothyronine (thyroxine or T4) and by 3,3',5-triiodothyronine (T3).Selenium is the second heaviest element found covalently bound to carbon, but it has a much broader physiological role than iodine.Of note, the activation of T4 to T3, by 5'-deiodination, is catalyzed by the selenoenzyme iodothyronine deiodinase (Dio).The catalysis involves a direct interaction of selenium (as selenol group) with iodine 27,28,35,36 (Table 4).The role played by selenium in life is much narrower than sulfur, which is found adjacent to selenium in the periodic table.[28][29][30]38 As cited above, the human genome encodes only 25 selenoproteins and the maximum number of selenoproteins found in vertebrates is about 40. 45The limited participation of selenium in the physiology of living cells indicates that the selenol group was selected by evolutional pressures to perform only a restricted set of chemical reactions.The restricted role of selenium in living cells and the negligible occurrence of the selenol group out of selenoproteins is certainly determined by the extreme reactivity of the selenol groups under physiological conditions. 46,47The unstable nature of the selenol group found in low molecular mass molecules has been recognized for a long time in pure chemical systems. 48In fact, the stabilisation of the selenol group can only be accomplished in the complex microenvironment of high molecular mass selenoproteins. 282][43] Consequently, one important question about the physiological chemistry of selenium is: why have some types of living cells been selected to have selenium as a part of their biochemical machinery?
Selenium (-SeH/-Se -) can be a better nucleophile than sulfur (-SH/-S -) in living cells and, consequently, catalyze more efficiently the reduction of biologically relevant chemical species, 38,40,62 but this does not explain its absence in a large number of living organisms.Since the molecular logic of the living state assumes that living cells obey the principle of maximal economy, 63 we can presume that the intricate complexity associated with the incorporation of selenium into the backbone of selenoproteins brought some adaptive biochemical properties that cannot be understood in simple redox chemical terms. 64The apparent conundrum about why selenium is essential only to some but not all living cells will require much more refined studies of the biochemical properties of the thiol and selenol groups of proteins from archea to mammals.
The objective of this minireview, compiled to mark the 80 th anniversary of Dr. Jacek Młochowski, who was a researcher dedicated to the synthesis of organoselenium compounds and one of the pioneers of research into their use as potential pharmacological agents, [65][66][67][68][69][70][71][72][73][74][75][76][77][78][79][80] is not to offer the reader a comprehensive account of the importance of selenium in organic synthesis of pharmacologically active compounds, because this can be found in several comprehensive reviews. 70, Here e give a brief historical account of the biological interest in selenium and its use in the synthesis of organoselenium compounds as biologically active molecules.We will discuss some critical points in the field that we realize are delaying the development of therapeutically effective agents.In terms of cell biology, we will present, in a brief way, the history of the biological interest in the element selenium, particularly how its incorporation into organic moieties has been important to life from a toxicological, pharmacological and physiological point of view.Although thousands (perhaps millions) of organoselenium compounds have already been synthesized since 1836; 70 only a small portion of them have been tested in biological systems.In our opinion, the absence of high-throughput methods to investigate the toxicity of the countless number of newly synthesized organoselenium compounds on rational grounds is the main bottleneck to the development of organoselenium therapeutic agents.The critical and elegant review: "Organochalcogen as peroxidase mimetics as potential drugs: a long story of a promise still unfulfilled" by Orian and Toppo 111 has recently addressed the necessity of new approaches to study the biology of organochalgogens.The development of in silico models to predict both thermodynamic parameters of intermediates stability and the interaction of organoselenium compounds with specific molecular targets are highly needed.Though the papers dealing with in silico simulation of organoselenium compounds chemical and biochemical behavior have been increasing, [111][112][113][114][115][116][117][118][119][120][121] we are far from accomplishing the task.

A brief history of the synthesis of organoselenium compounds
Interest in the synthesis of organoselenium compounds goes back to the early 19 th century, when the first organoselenium compound was synthesized.According to Fredga, 122 organoselenium chemistry started on January 23, 1847, precisely, when Wöhler wrote to Berzelius: "Today a small grandchild of yours has come in the world, a child of selenium, the selenomercaptan".The synthesized molecule was ethyl selenol (Table 5), prepared from calcium ethyl sulfate and potassium hydroselenide by Siemens.Siemens reacted the product of the reaction with Hg(II) to form CH3CH2SeHgSeCH2CH3 and proved that it was a "selenomercaptan". 123owever, according to Prof. Młochowski and collaborators, the synthesis of diethylselenide has priority, being reported in 1836 by Löwing. 70n Table 5 are listed the first selenium compounds synthesized by man and the first compound detected in mammalian metabolism by its smell.The ethyl-containing molecules were synthesized by man, 70 whereas the dimethylselenide was discovered by the sense of smell of the early toxicologists, who evaluated the toxicity of inorganic selenium salts in mammals (see below in Section 3.1).The book "The organic compounds of tellurium and selenium belonging to the alcohol series" by Dean, 124 who had worked in the laboratory of Wöhler, emphasizes the similarity between S, Se, and Te.By paraphrasing the author: "In regard to the history of these compounds it may be observed, that the extraordinary resemblance between sulfur, selenium, and tellurium first led to the idea of substituting selenium or tellurium for sulfur in various organic compounds…", we can realize that at a very early stage of organic synthesis development, selenium was being investigated as surrogate for sulfur.As briefly commented in the first section of this mini-review, biological evolution has apparently endeavored to substitute sulfur by selenium at an early stage of the evolution of a living cell.With the introduction of selenium in the biochemical machinery, the living cell gained efficiency in metabolizing important redox reactions, 5,17,38,40 whereas the synthetic chemist gained versatility in their reactions. 65,70, One iortant limitation in the early years of organoselenium compound synthesis was the availability of selenium. 124Regardless of the unavailability of the element in the 19 th century, the field progressed considerably.In his classical review from 1941, Edgar P. Painter stated that hundreds of organoselenium compounds had been described and presented a list of the available synthetic strategies to prepare diselenides, selenols, selenenic, and selenonic acids, among others. 48Recent literature considers that modern era of organoselenium synthesis began in the seventies of the last century. 1,702][83][84][85][86][87][88][89][90][91][92][93][94][95][96][97][98][99] The importance of this element in the field of organic synthesis can be verified by the vast literature on organoselenium chemistry.From Painter's time to the present, we can roughly estimate the number of new organoselenium compounds synthesized every year and now thousands to millions of compounds can be found in the literature.

Re-discovery of Ebselen: can the chemist imitate chemistry of life?
6][127] Ebselen was originally synthesized in 1924 by Lesser and Weiss 128 and it was re-discovered as an antioxidant about 40-50 years ago (for an interesting testimony about the development of Ebselen as a therapeutic agent see refs 129,130) (Table 6).[127]  The use of Ebselen in clinical trials to treat brain pathologies associated with oxidative stress changed the view of selenium compounds.As stated in their vivid report about the steps that led to the development of Ebselen as therapeutic agent, the first goal was to use Ebselen as a source of selenium for the synthesis of selenoproteins.The discovery of selenium in the structure of gluthathione peroxidase (GPx) and its role in peroxide degradation 131,132 had introduced the idea that selenium supplementation could be an important strategy to improve the activity of GPx.The rationale that Ebselen could be a better source of selenium than inorganic selenium or naturally occurring organic selenium forms can be easily understood by the fear of selenium (selenophobia) that was prevalent in the 20 th century 133 (the history of selenium toxicity to mammals will be presented in the next section of this review).Contrary to the expectation of the researchers, 129 the selenium in Ebselen was not metabolized to the inorganic selenium pool, which would be required for selenium incorporation into selenocysteine (i.e., into selenoproteins; 5 Figure 1).6][137] The basic mechanism of peroxide decomposition by organoselenium compounds (e.g.Ebselen and diselenides, Table 6), which forms selenol intermediates, 101,104 is supposed to be similar to that of the native GPx isoforms (Scheme 1).Other selenium compounds that are not metabolized to selenol intermediates (for instance, tetrahydroselenophenes, Table 6) can also mimic the activity of GPx by forming a selenoxide intermediate after decomposing peroxides. 81heme 1. Reduction of Ebselen and diphenyl diselenide to selenol intermediates.Reaction 1 is catalyzed by thioredoxin reductase (TrxR) and uses NAPDH as an electron donor.Reaction 2 uses glutathione (GSH) as an electron donor.
Ebselen and diphenyl diselenides can be reduced to selenol intermediates both after reacting directly with thiol-containing molecules or after being reduced by TrxR [138][139][140] (Scheme 1).Literature studies have also indicated that organoselenium compounds can interact with specific thiol-containing proteins. 141,142Ebselen and diphenyl diselenide have been reported to inhibit the activity of important metabolic enzymes in vitro, for instance, Na + ,K + -ATPase, 101,104,108 but there is no evidence that they can target it after in vivo administration.The inhibition of such a crucial enzyme can have unpredictable toxicological effects, particularly to the central nervous system.The specific interaction of Ebselen with inositol monophosphatase (IMPase) has been suggested to be involved in the lithium mimetic activity of Ebselen 143 and can be involved in the antidepressive activity of Ebselen in experimental models. 144In contrast, diphenyl diselenide, which exhibited similar antidepressive activity in rodent models, is not an inhibitor of IMPase. 143The oxidation of thiol-containing proteins by organoselenium compounds can be involved both in their pharmacological and toxicological effects.The chemical mechanism of oxidation of thiol-containing proteins by organochalcogens is similar to that presented in Table 4 (reaction 1), where the thiol group of the protein substitute GSH as reducing agent.Theoretically, the oxidation of a therapeutically important protein by an organochalcogen can both cause a specific physiological effect and can generate the selenol intermediates.The selenol intermediate can then decompose peroxides and exhibit antioxidant properties.
1][152][153][154][155][156][157] For instance, the oxidation of Keap-1 by organoselenium compounds can activate the Nrf-2-transcript factor that stimulates the expression of antioxidant proteins.In summary, the pharmacological or toxicological effects of organoselenium compounds include the oxidation of thiol groups and from the chemical point of view, the basic mechanisms of reaction are similar to those presented by the selenoproteins.

Early history of inorganic selenium toxicology
0][161][162][163][164][165] According to Charles Jones, 159 the first author to study the toxicity of selenium salts in animals was C. G. Gmelin.However, Moxon and Morris, 3 in their classical review on "selenium poisoning", argued that they could not find experiments related to the toxicity of selenium in Gmelin's book.Gmelin studied the toxicity of tellurium salts 160 and the source of confusion can be derived from the similar chemical and biochemical behavior of selenite and tellurite in bacteria and mammals, i.e., both salts can be reduced to elemental forms. 156,159,164,165Jones stated that Hasen (1853) described selenium granules deposited in the organs of animals injected with selenite and identified a garlic-like odor in the breath of poisoned animals; but apparently Hansen injected tellurite and not selenite.The chemical nature of the volatile compound(s) with garlic odor was supposed to be either dimethyl selenide or diethyl selenide 159 (in fact, dimethyl telluride or diethyl telluride).In 1909, Jones demonstrated the deposition of a brick-red precipitate in the organs of animals intoxicated with selenite, but he concluded that the formation of elemental selenium was not the cause of the death of intoxicated animals, 159 because he could observe comparable selenium granules in animals treated with high but non-lethal doses of selenite.The participation of hydrogen selenide (i.e., H2Se) as a toxic metabolite of Se(IV) or Se(VI) was not considered by Jones, though hydrogen selenide was known to cause some signs of intoxication similar to those caused by lethal intoxication with selenite. 158,159ones also observed a garlic-like odor in the breath of poisoned mammals.The emanation of garlic-like odor could also be detected in the organs isolated from animals poisoned with Na2SeO3.The proposal that the garlic-like odor present in the breath of selenium-poisoned animals was produced by dimethyl selenide was made by Hofmeister. 159,165He injected sodium tellurite and detected the odor similar to dimethyl sulfide and postulated that exhaled compound after administration of tellurite was dimethyl telluride.Based on the chemical similarity between selenium and tellurium, Hofmeister speculated that the garlic-like odor detected after selenite intoxication was produced by dimethyl selenide. 165However, a conclusive demonstration that inorganic selenium exposure was associated with dimethyl selenide exhalation was made by McConnell and collaborator. 165The research work concerning the toxicity of selenium and tellurium salts carried out in the second half of the 19 th century were crucial to establish the first metabolic pathway of selenium in mammals (Scheme 2).The elucidation of the chemical nature of the first metabolically produced organoselenium molecule was confirmed only at the end of the second half of the 20 th century. 164,1655][166][167][168] Of particular importance to the physiological chemistry of selenium, selenide (Se 2-) is the unique chemical form of selenium metabolized to selenophosphate (HPSeO3 2-or selenophosphoric acid, H3PSeO3), which can then be incorporated into the backbone of selenocysteine. 5The formation of elemental selenium was evidenced by the formation of brick-red selenium precipitate in the organs of animals intoxicated with selenium salts.The formation of organic selenium (dimethylselenide) was hypothesized by analogy with tellurite metabolism and by sensorial identification of a garlic-like odor in the breath of living intoxicated animals or by the release of garlic odor from their isolated organs.The formation of dimethylselenide was dissociated from the formation of Se(0) by Jones. 159He demonstrated that heating the organs up to 60 o C suppressed the formation of the garlic odor [(CH3)2Se], but did not stop the formation of elemental Se.Jones concluded that carbohydrates, for instance, glucose reduced the selenite to elemental selenium.However, nowadays we know that the thiol group from glutathione and proteins are responsible by the chemical reduction of selenite to elemental selenium.

Oxidation of thiol groups and toxicity of inorganic selenium forms
Our understanding of the toxicology of inorganic selenium is still elusive, however the oxidation of thiolcontaining molecules by cationic selenium ions is involved in the process of toxicity as macroscopically demonstrated by the early toxicologists. 158,159The reduction of Se(IV) and Se(VI) to elemental selenium by isolated organs from animals poisoned with selenite, forming brick-red precipitates, was possible even at high temperatures. 159This observation strongly indicates that selenate and selenite can be chemically reduced to elemental selenium (i.e., without the participation of heat-sensitive enzymes).Accordingly, selenite can oxidize GSH 169 and the depletion of this important antioxidant molecule may cause toxic consequences to cells. 170,171[178][179][180]

The therapeutic use of inorganic selenium
2][183] According to Watson-Williams, 181 the rationale for administering selenium suspensions to human was based on the previous successful use of the element in animal models of cancer.Additionally, Watson-Williams 181 stated that "injections of colloidal selenium had been used in human patients with inoperable carcinoma" in France.][183] Nonetheless, the use of colloidal selenium was abandoned, possibly because its efficacy was not confirmed and/or in view of the increasing awareness of the toxicology of selenium at those times (see for instance refs 3  and 133).The fear of the element, which had escalated around the '30s to '60s, was so prevalent that Frost and Olso 133 coined the term "selenophobia" to express the negative view of selenium.
The therapeutic use of elemental selenium in patients indicated that Se(0) was well tolerated by humans after systemic administration.Contrasting with the results obtained with Se(IV) and Se(VI), which indicated extreme toxicity in mammals, 159 elemental selenium seemed to be relatively inert in humans.5][186][187][188][189] However, the main results of an extensive study, in which selenomethionine and vitamin E were used as nutritional supplements, did not indicate a protective role for selenium against cancer. 190,191In contrast, some meta-analysis studies have supported a protective role of selenium in some types of cancer. 192,1938][199][200] However, there are also indications that high levels of toenail Se is associated with a lowered risk of developing type 2 diabetes. 201,202.4 The toxicology of naturally occurring organoselenium compounds: selenium as a causative factor of livestock poisoning At the same time that elemental selenium was being used clinically to relieve the malady associated with different types of inoperable carcinomas, [181][182][183] researchers in the USA were revealing the central role of "naturally-occurring selenium" as the causative factor of alkali disease in livestock.3,48,[203][204][205][206] The studies of Franke and collaborators demonstrated that grains grown in regions previously associated with alkali disease had an elevated level of selenium and showed that the extraction of selenium from the plants abolished their toxicity.[203][204][205][206] Franke and collaborators were also the pioneers in demonstrating that selenite could reproduce the majority of toxic symptoms caused by the ingestion of plants containing high levels of selenium.Additionally, Franke and Painter 193 207-211 found that the removal of selenium from toxic protein hydrolysates abolished the toxicity of wheat protein.Some important chemical forms of naturally occurring Se are presented in Tables 1 and 2. In the case of the Franke and Painter studies, [209][210] the main toxic form can retrospectively be ascribed to an organic form of selenium, for instance, selenomethionine and methylselenocysteine. 212,213 Selenium accumulator plants can synthesize different organoselenium compounds, which can have toxicological properties, but little is known about the metabolism and toxicity of different combination of organoselenium compounds found in grain and vegetables.
5][216][217][218] Here we should point out that keratin proteins, which are the key structural components of hair, hooves and nails, have a high cysteine content. 219The disruption of keratin-containing hair, hooves and nails by selenium intoxication demonstrate a tropism of selenium metabolites in cells that synthesize proteins rich in cysteine.The incorporation of selenium in secretions from keranocytes can be attested by the high content of the element in hair and nail. 220,221lthough the toxicity of naturally occurring organoselenium compounds is not coincident with that of the inorganic salts, the oxidation of low and high molecular mass thiol molecules can also be involved in their toxicity.However, our knowledge about the selenium metabolites involved in the oxidative toxicity of different naturally occurring selenium compounds is still elusive.[176] 7). 227The authors stated that selenium compounds appeared interesting to be tested in view of their relationship with sulfur metabolism; however, the authors assessed the distribution of selenium, but did not present data about the potential anti-tumor effect of the different compounds tested.9][230][231] Though this class of compound has been shown to exhibit beneficial effects in vitro and in vivo against cancer (Table 7), they were not put through clinical trials.In addition to the potential anticancer properties, organoselenium compounds have been shown to exhibit interesting pharmacological properties in different experimental models of human pathologies.Of note, they can have antioxidant, neuroprotective, anti-inflammatory, antidepressive, imunomudulatory, antimicrobial activity against pathogenic bacteria, fungi and viruses, among others 14,[232][233][234][235][236][237][238][239] .

The Biological and Pharmacological Interest in Synthetic Organoselenium
The effectiveness of some organoselenium compounds as beneficial agents in quite different acute or chronic models of human degenerative disease can be explained by their anti-inflammatory and antioxidant properties.0][241][242][243][244][245][246][247][248][249] The inflammatory processes stimulate the over production of reactive species (e.g.reactive oxygen (ROS) and nitrogen species(RNS)) and these will further stimulate the release of pro-inflamatory molecules (Scheme 3).Although Scheme 3 is an oversimplification of a rather complicated processes, the modulation of peroxide tonus by organoselenium compounds can decrease the release of pro-inflammatory mediators indirectly.A direct anti-inflammatory role of some organochalcogens is also possible plausible, but this has not yet been investigated in detail.The fact that organoselenium compounds exhibit protective effects in a variety of models of diseases with different etiologies argues in favor of quite general mechanism of action.The GPx-like or thiol peroxidase-like activity can explain the general beneficial effects of organoselenium compounds.For the case of Ebselen and diphenyl diselenide, the GPx-like activity is thought to be mediated by the formation of selenol intermediates.In view of the simple structure of the organoselenium compounds cited above we can predict that the selenol intermediates formed will not present selectivity.The selenol intermediates will be able to mimic the physiological chemistry of selenoproteins in general, and not only of GPx isoforms.However, we have only a vague idea about the efficacy of organochalcogens to mimic specific selenoproteins.Another important mechanism that seems to be involved in the beneficial effect of organochalcogens is their capacity to function as weak electrophiles.103][104][105][106][107][108][109][110][150][151][152][153][154][155][156][157] Here we discuss one important mechanism that has been neglected in the recent literature about the beneficial effects of some synthetic organoselenium compounds.The studies in question were published about 60 years ago and just one year after the appearance of the most well-known paper about the biological importance of selenium. 250,251The proximity of the two publications of Schwartz and Foltz obscured the importance of the second study.In this classical study from 1958, Schwarz and Foltz clearly demonstrated that different mono-and diselenides prevented dietary liver necrosis in rats fed with a vitamin E deficient diet. 2501][252][253] These studies provided the first experimental demonstration that both inorganic and organic selenium compounds had hepatoprotective effects in rodents.Taken together with the study of Adams and collaborators, 254 which showed the release of selenium from the organic moiety of diphenyl diselenide, the studies of Schwartz and collaborators 250,255 indicated that simple organoselenium compounds such as aliphatic diselenides, diphenyl diselenides and diphenyl selenides can furnish selenium to the inorganic pool of selenium.The inorganic selenide released from aromatic and aliphatic selenides can be used in the synthesis of selenoproteins.Thus, in part, the beneficial effects of organoselenides can be related to modulation of selenoprotein synthesis.
29,130 Ebselen is now included in the chemical library of the National Institutes of Health Clinical Collection as a safe drug, 143 but without a target pathology to treat.The interest in finding a target for Ebselen has increased considerably in the last ten years and it is now being studied as a potential lithium mimetic in humans, 143,[256][257][258][259] experimentally as an antimicrobial agent, [260][261][262][263][264] among others. 265][268][269][270][271][272][273] A brief historical account of the biological timeline of selenium is presented below (Table 8).

Concluding Remarks and Perspectives
The biological interest in selenium has paralleled that of organoselenium compounds before and after the recognition that selenium has physiological roles in some forms of life.Nowadays, the great challenge of organoseleno synthetic chemists is to create molecular moieties capable of imitating the physiological chemistry of selenium (i.e., selenoproteins) without being toxic to the living cells.Optimistically, the new compounds will need to have selectivity and catalyze specific reactions.This will be an advance over contemporary organoselenium compounds that can imitate non-specifically the reactions catalyzed by different isoforms of selenoenzymes.Here it is also important to emphasize that the development of selenium-containing molecules that can selectively interact with specific thiol-containing proteins will also be of great therapeutic value.The development of molecules that could delivery selenium preferentially to specific tissues or groups of cells can also be important to the treatment of cancer and other pathologies.The tasks are hard, but the field is slowly moving forward.The bottlenecks here are: 1) the absence of in silico methods to predict the chemical, biochemical and toxicological behavior of new molecules; 2) the limitation of in vitro methods to study the toxicological and biochemical properties of a large number of molecules (i.e., the available methods have limited capacity of predicting either the toxicity or the beneficial effects of new organoselenium compounds and 3) the limited number of compounds that have been studied in some detail from the biological point of view.Thus, it seems more reasonable to select some promising classes of compounds and test them mechanistically than to test a vast number of molecules in complex biological systems empirically.The probability of finding a target for an organoselenium compound by chance is very small.The use of available in silico methods can increase the chance of successful discovery of drugs with real therapeutic potential.Consequently, the in depth study of existing in silico methodologies and their improvement will be decisive for the future of the field of organoselenium compounds.

Figure 1 .
Figure 1.Structure of the naturally occurring amino acids with selenol, thiol and hydroxy groups.

Figure 2 .
Figure 2. Essential elements in mammalian cell physiology.

Scheme 2 .
Scheme 2. Formation of Se(0) and volatile organic selenium after reduction of selenate or selenite in living cells.

Table of Contents 1 .
Introduction 1.1 The selenol group: the softest of the nucleophiles in cell biology 1.2 The essentiality of selenium to life 2.Chemical Interest in the Use of Selenium in Organic Synthesis 2.1 A brief history of the synthesis of organoselenium compounds 2.2 Re-discovery of Ebselen: can the chemist imitate chemistry of life? 3. Biological Interest in Selenium: a Brief Historical Account 3.1 Early history of inorganic selenium toxicology 3.2 Oxidation of thiol groups and toxicity of inorganic selenium forms 3.3 Therapeutic use of inorganic selenium 3.4 Toxicology of naturally occurring organoselenium compounds: selenium as a causative factor of livestock poisoning 4. The Biological and Pharmacological Interest in Synthetic Organoselenium Compounds: an Overview 4.1 The early pharmacological and nutritional use of synthetic organoselenium compounds 5. Concluding Remarks and Perspectives References

Table 1 .
Inorganic and simple organic forms of selenium found in the environment and/or in living cells

Table 2 .
Amino acids-, amino acid derivatives-, peptides-, and protein-containing selenium found in plants and/or mammals

Table 3 .
Some biological and chemical properties of the lateral groups of the amino acids selenocysteine,

Table 5 .
Early historical landmarks in selenium chemistry

Table 6 .
The Structure of some organoselenium compounds with interesting pharmacological properties

Table 8 .
Timeline of selenium in science